Article
Rapid assay of A2058T-mutated 23S rRNA allelic profiles associated with high-level macrolide resistance in Moraxella catarrhalis.
Journal of medical microbiology - 1 Sept 2015
Saito Ryoichi, Kasai Ayako, Ogihara Shinji, Yamada Kageto, Tao Kazuyuki
Abstract excerpt
We report on a restriction fragment-length polymorphism (HpyCH4III) assay for profile analysis of 23S rRNA gene A2058T-mutated alleles associated with high-level macrolide resistance in Moraxella catarrhalis. Our assay results were supported by DNA sequencing analysis, allowed for simultaneous testing of many strains, and produced results from pure-cultured colonies within 4 h.
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