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Article

Flexibility of NS5 Methyltransferase-Polymerase Linker Region Is Essential for Dengue Virus Replication.

Journal of virology - 1 Oct 2015

Zhao Yongqian, Soh Tingjin Sherryl, Chan Kitti Wing Ki, Fung Sarah Suet Yin, Swaminathan Kunchithapadam, Lim Siew Pheng, Shi Pei-Yong, Huber Thomas, Lescar Julien, Luo Dahai, Vasudevan Subhash G

Abstract excerpt

We examined the function of the conserved Val/Ile residue within the dengue virus NS5 interdomain linker (residues 263 to 272) by site-directed mutagenesis. Gly substitution or Gly/Pro insertion after the conserved residue increased the linker flexibility and created slightly attenuated viruses. In contrast, Pro substitution abolished virus replication by imposing rigidity in the linker and restricting NS5's...

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Identifiers

PMID: 26269182
DOI: 10.1128/JVI.01239-15
PMCID: PMC4580200

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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