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Article

Impact of Detachment Methods on M2 Macrophage Phenotype and Function.

Journal of immunological methods - 1 Nov 2015

Chen Shaodong, So Edward C, Strome Scott E, Zhang Xiaoyu

Abstract excerpt

The methods of cell detachment influence phenotype and function of human macrophages cultured in vitro. However, comparative studies defining the influence of cell detachment techniques on secondary characterization of M1 or M2 polarized macrophages are largely absent from the literature. In this study we evaluated the impact of trypsin, accutase, EDTA, PBS, and cell scraping on: A. cell recovery, B. phenotype...

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Identifiers

PMID: 26253940
DOI: 10.1016/j.jim.2015.08.001

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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