Article
The Culture Dish Surface Influences the Phenotype and Dissociation Strategy in Distinct Mouse Macrophage Populations.
Frontiers in immunology - 1 Jan 2022
Song Qiaoling, Zhang Yazhuo, Zhou Mingming, Xu Yuting, Zhang Qianyue, Wu Lihong, Liu Shan, Zhang Minghui, Zhang Lei, Wu Zhihua, Peng Weixun, Liu Xutao, Zhao Chenyang
Abstract excerpt
The nature of the culture dish surface and the technique used to detach adherent cells could very likely influence the cell viability and cell membrane protein integrity of harvested macrophages. Several previous studies assessed the detachment efficacies of enzymatic and non-enzymatic methods for harvesting the single cell suspensions of macrophages, but a comprehensive study assessing different dissociation...
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