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Article

An improved fluorescence polarization assay in 5'-nuclease reaction for gene promoter methylation detection.

Journal of biotechnology - 10 Oct 2015

Su Haichuan, Wu Zhongliang, Wang Yukun, Jiang Yinghao, Qiang Shaoying, Cheng Hong, Liu Wenchao, Zhang Ju

Abstract excerpt

The detection of gene promoter methylation plays increasing roles in personalized medicine. In this study, an improved gene promoter methylation assay based on fluorescence polarization in 5'-nuclease reaction was developed. The novel assay offered a homogeneous annealing and cleavage reaction fully integrated with PCR which used a probe labeled with fluorescence without quencher to obtain the decreased...

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PMID: 26197420
DOI: 10.1016/j.jbiotec.2015.07.003

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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