Article
Mutation in galP improved fermentation of mixed sugars to succinate using engineered Escherichia coli AS1600a and AM1 mineral salts medium.
Bioresource technology - 1 Oct 2015
Sawisit Apichai, Jantama Kaemwich, Zheng Huabao, Yomano Lorraine P, York Sean W, Shanmugam Keelnatham T, Ingram Lonnie O
Abstract excerpt
Escherichia coli KJ122 was engineered to produce succinate from glucose using the wild type GalP for glucose uptake instead of the native phosphotransferase system (ptsI mutation). This strain now ferments 10% xylose poorly. Mutants were selected by serial transfers in AM1 mineral salts medium with 10% xylose. Clones from this population all exhibited a similar improvement, co-fermentation of an equal mixture of...
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