Article
Multiplex growth rate phenotyping of synthetic mutants in selection to engineer glucose and xylose co-utilization in Escherichia coli.
Biotechnology and bioengineering - 1 Apr 2017
Groot Joost, Cepress-Mclean Sidney C, Robbins-Pianka Adam, Knight Rob, Gill Ryan T
Abstract excerpt
Engineering the simultaneous consumption of glucose and xylose sugars is critical to enable the sustainable production of biofuels from lignocellulosic biomass. In most major industrial microorganisms glucose completely inhibits the uptake of xylose, limiting efficient sugar mixture conversion. In E. coli removal of the major glucose transporter PTS allows for glucose and xylose co-consumption but only after...
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