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Article

Multiplexed highly-accurate DNA sequencing of closely-related HIV-1 variants using continuous long reads from single molecule, real-time sequencing.

Nucleic acids research - 16 Nov 2015

Dilernia Dario A, Chien Jung-Ting, Monaco Daniela C, Brown Michael P S, Ende Zachary, Deymier Martin J, Yue Ling, Paxinos Ellen E, Allen Susan, Tirado-Ramos Alfredo, Hunter Eric

Abstract excerpt

Single Molecule, Real-Time (SMRT) Sequencing (Pacific Biosciences, Menlo Park, CA, USA) provides the longest continuous DNA sequencing reads currently available. However, the relatively high error rate in the raw read data requires novel analysis methods to deconvolute sequences derived from complex samples. Here, we present a workflow of novel computer algorithms able to reconstruct viral variant genomes present...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 26101252
DOI: 10.1093/nar/gkv630
PMCID: PMC4787755

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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