Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Design of an ectoine-responsive AraC mutant and its application in metabolic engineering of ectoine biosynthesis.

Metabolic engineering - 1 Jul 2015

Chen Wei, Zhang Shan, Jiang Peixia, Yao Jun, He Yongzhi, Chen Lincai, Gui Xiwu, Dong Zhiyang, Tang Shuang-Yan

Abstract excerpt

Advanced high-throughput screening methods for small molecules may have important applications in the metabolic engineering of the biosynthetic pathways of these molecules. Ectoine is an excellent osmoprotectant that has been widely used in cosmetics. In this study, the Escherichia coli regulatory protein AraC was engineered to recognize ectoine as its non-natural effector and to activate transcription upon...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Directed Molecular Evolution
Share to Escherichia coli

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Escherichia coli Proteins

Share to Metabolic Engineering

Share to Mutation

Source

Identifiers

PMID: 26051748
DOI: 10.1016/j.ymben.2015.05.004

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Design of an ectoine-responsive AraC mutant and its application in metabolic engineering of ectoine biosynthesis.PMID 26051748Read ↗

AraC regulatory protein mutants with altered effector specificity.Semantic proximity Read ↗

A novel DMAPP-responding genetic circuit sensor for high-throughput screening and evolving isoprene synthase.Semantic proximity Read ↗

Product-induced gene expression, a product-responsive reporter assay used to screen metagenomic libraries for enzyme-encoding genes.Semantic proximity Read ↗

Elucidating residue roles in engineered variants of AraC regulatory protein.Semantic proximity Read ↗

Engineering 2,4-Diacetylphloroglucinol-Hyper-Tolerant Escherichia coli via Adaptive Evolution for Efficient Biosynthesis and an Organic Solvent-Free Downstream Process.Semantic proximity Read ↗

A regulatory toolkit of arabinose-inducible artificial transcription factors for Gram-negative bacteriaSemantic proximity Read ↗

Manipulating the expression rate and enantioselectivity of an epoxide hydrolase by using directed evolution.Semantic proximity Read ↗

Molecular engineering of the salicylate-inducible transcription factor Sal7AR for orthogonal and high gene expression in Escherichia coli.Semantic proximity Read ↗

Engineering an allosteric transcription factor to respond to new ligands.Semantic proximity Read ↗

Structure and function of the macrolide biosensor protein, MphR(A), with and without erythromycin.Semantic proximity Read ↗

Biochemical characterization and mutational analysis of the tetrameric DABA transaminase EctB from the Arctic bacterium Marinobacter sp. CK1.Semantic proximity Read ↗

Using Data Science for Mechanistic Insights and Selectivity Predictions in a Non-Natural Biocatalytic Reaction.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.