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Article

Modified primers for rapid and direct electrochemical analysis of coeliac disease associated HLA alleles.

Biosensors & bioelectronics - 15 Nov 2015

Joda Hamdi, Beni Valerio, Willems Andreas, Frank Rainer, Höth Julian, Lind Kristina, Strömbom Linda, Katakis Ioanis, O Sullivan Ciara K

Abstract excerpt

Direct detection of PCR product via hybridisation assay, would facilitate the development of rapid tools for genetic analysis. Here, a PCR primer designed to generate a PCR amplicon tagged with single stranded DNA tails at each end of the duplex, which can be used for direct hybridisation with a surface immobilised probe and an enzyme labelled reporter probe is presented. Four modified sequence specific primers...

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Identifiers

PMID: 26043316
DOI: 10.1016/j.bios.2015.05.048

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 5 Sept 2026. Manifest publication-graph-cohort-2026-09-05.1.

Source receipt: pubmed, retrieved 5 Sept 2026.

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