Article
Visual genotyping of thalassemia by using pyrrolidinyl peptide nucleic acid probes immobilized on carboxymethylcellulose-modified paper and enzyme-induced pigmentation.
Mikrochimica acta - 18 Mar 2020
Jirakittiwut Nuttapon, Munkongdee Thongperm, Wongravee Kanet, Sripichai Orapan, Fucharoen Suthat, Praneenararat Thanit, Vilaivan Tirayut
Abstract excerpt
A simple probe pair was designed for the detection of hemoglobin E (HbE) genotype, a single-point mutation that leads to abnormal red blood cells commonly found in South East Asia. The key to differentiation is the use of a conformationally constrained peptide nucleic acid (PNA) that was immobilized on carboxymethylcellulose-modified paper. This was then used for target DNA binding and visualization by an...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.