Article
A new target region for changing the substrate specificity of amine transaminases.
Scientific reports - 1 Jun 2015
Guan Li-Jun, Ohtsuka Jun, Okai Masahiko, Miyakawa Takuya, Mase Tomoko, Zhi Yuehua, Hou Feng, Ito Noriyuki, Iwasaki Akira, Yasohara Yoshihiko, Tanokura Masaru
Abstract excerpt
(R)-stereospecific amine transaminases (R-ATAs) are important biocatalysts for the production of (R)-amine compounds in a strict stereospecific manner. An improved R-ATA, ATA-117-Rd11, was successfully engineered for the manufacture of sitagliptin, a widely used therapeutic agent for type-2 diabetes. The effects of the individual mutations, however, have not yet been demonstrated due to the lack of experimentally...
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