Article
Dominant Suppression of Inflammation via Targeted Mutation of the mRNA Destabilizing Protein Tristetraprolin.
Journal of immunology (Baltimore, Md. : 1950) - 1 Jul 2015
Ross Ewan A, Smallie Tim, Ding Qize, O'Neil John D, Cunliffe Helen E, Tang Tina, Rosner Dalya R, Klevernic Iva, Morrice Nicholas A, Monaco Claudia, Cunningham Adam F, Buckley Christopher D, Saklatvala Jeremy, Dean Jonathan L, Clark Andrew R
Abstract excerpt
In myeloid cells, the mRNA-destabilizing protein tristetraprolin (TTP) is induced and extensively phosphorylated in response to LPS. To investigate the role of two specific phosphorylations, at serines 52 and 178, we created a mouse strain in which those residues were replaced by nonphosphorylatable alanine residues. The mutant form of TTP was constitutively degraded by the proteasome and therefore expressed at...
Topics
- Alanine
- Amino Acid Substitution
- Animals
- Cell Line
- Cytokines
- Female
- Gene Expression
- Inflammation
- Lipopolysaccharides
- Macrophages