Article
A new method for ABO genotyping using a multiplex single-base primer extension reaction and its application to forensic casework samples.
Legal medicine (Tokyo, Japan) - 1 Oct 2004
Doi Yusuke, Yamamoto Yuji, Inagaki Sachiyo, Shigeta Yoshiaki, Miyaishi Satoru, Ishizu Hideo
Abstract excerpt
We developed a new method for ABO genotyping using a multiplex single-base primer extension reaction. The method allows for the simultaneous detection of six SNP sites in the ABO gene (nt 261, 297, 681, 703, 802, and 803) and the determination of ABO genotypes from their combinations. It enabled ABO genotyping of all samples of peripheral blood DNA extracted from 103 Japanese individuals, and had a highly...
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