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Article

Crop plant genotyping by real-time PCR analysis of crude extracts of seeds.

Journal of AOAC International - 1 Jan 2000

Lan Yang, Wang Cheng-En, Wu Chuan-Fang, Liu Zhong-Yu, Gao Chun-Fang

Abstract excerpt

Development of agricultural biotechnology requires rapid and convenient methods for crop plant genotyping. Real-time PCR is sensitive and reliable, and has been a routine technique in plant research. However, its application is limited by the cumbersome DNA template preparation procedures. We tested three PCR master mixes for direct amplification of crude seed DNA extracts without extensive purification. One mix...

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PMID: 25857871
DOI: 10.5740/jaoacint.14-104

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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