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Non-destructive high-throughput DNA extraction and genotyping methods for cotton seeds and seedlings.

BioTechniques - 1 May 2015

Zheng Xiuting, Hoegenauer Kevin A, Maeda Andrea B V, Wang Fei, Stelly David M, Nichols Robert L, Jones Don C

Abstract excerpt

Extensive use of targeted PCR-based genotyping is precluded for many plant research laboratories by the cost and time required for DNA extraction. Using cotton (Gossypium hirsutum) as a model for plants with medium-sized seeds, we report here manual procedures for inexpensive non-destructive high-throughput extraction of DNA suitable for PCR-based genotyping of large numbers of individual seeds and seedlings. By...

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PMID: 25967902
DOI: 10.2144/000114286

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