Article
A simplified genomic DNA extraction protocol for pre-germination genotyping in rice.
Genetics and molecular research : GMR - 11 Jun 2015
Duan Y B, Zhao F L, Chen H D, Li H, Ni D H, Wei P C, Sheng W, Teng J T, Zhang A M, Xue J P
Abstract excerpt
Genotyping is a critical step for molecular marker-assisted selection in rice. Rice genomic DNA samples for genotyping are typically isolated from living tissues such as seedlings. This requires the germination of all candidate seeds and extraction of DNA from the seedlings. Currently, an ideal individual is selected from a very large number of plants, which is time- and labor-consuming, requiring several...
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