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PCR Allele Competitive Extension (PACE) Workflow for Genotyping by SNP Identification.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2025

Torres Anthony, Argyris Jason, Gaudino Reginald

Abstract excerpt

This chapter presents protocols for advanced genotyping and genetic analysis in plant science, particularly utilizing DNA extracted from FTA samples and leaf tissue. PACE (PCR Allele Competitive Extension) enables high-throughput SNP genotyping with remarkable accuracy, specifically targeting and differentiating between two alleles of interest. The protocol involves using allele-specific primers and a PACE master...

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PMID: 40580292
DOI: 10.1007/978-1-0716-4642-7_12

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