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Article

Excised radicle tips as a source of genomic DNA for PCR-based genotyping and melting curve analysis in cotton.

Journal of biosciences - 1 Mar 2013

Rao P Srinivasa, Kumar P Sateesh, Sonti Ramesh V

Abstract excerpt

Genomic DNA isolation in cotton is complicated because of the presence of secondary metabolites that are inhibitory to PCR amplification. We report here that radicle tips, but not other parts of cotton seedlings, yield high-quality DNA that is readily amenable for PCR. The radicle-tip-excised seedlings retain viability because of the formation of adventitious roots. We demonstrate the utility of this method in...

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PMID: 23385824
DOI: 10.1007/s12038-012-9285-4

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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