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Cost-effective and reliable genomic DNA extraction from plant seedlings for high-throughput genotyping in seed industries.

Analytical biochemistry - 1 Sept 2023

Wanere Shyamkumar S, Phad Archana P, Jagtap Rameshwar K, Rawal Shuban K, Pyati Prashant S, Lomate Purushottam R

Abstract excerpt

Genetic purity of seeds is one of the critical aspects in the seed industry. Molecular seed testing laboratories are utilizing PCR based diagnostic tools for genetic purity analysis. High quality DNA is an essential prerequisite for such analyses. Here, we demonstrate a robust and inexpensive DNA extraction method to isolate genomic DNA from variety of crops. Current method (M2) was compared with four commonly...

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PMID: 37429485
DOI: 10.1016/j.ab.2023.115245

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