Article
A technical platform for PCR-based SNP screening in cereals and other crops.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2014
Wang Zining
Abstract excerpt
With the rapid development of sequencing technologies and sequenced genomes, single-nucleotide polymorphisms (SNPs) have become a common genomic tool in the study of biological diversity, genome variation, gene mapping, cloning, and marker-assisted selection. In this chapter, PCR-based SNP screening is discussed in detail. This includes preparation of solutions and buffers, designing of tetra-primers, PCR for DNA...
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