Article
Controlled expression of Drosophila homeobox loci using the Hostile takeover system.
Developmental dynamics : an official publication of the American Association of Anatomists - 1 Jun 2015
Javeed Naureen, Tardi Nicholas J, Maher Maggie, Singari Swetha, Edwards Kevin A
Abstract excerpt
BACKGROUND: Hostile takeover (Hto) is a Drosophila protein trapping system that allows the investigator to both induce a gene and tag its product. The Hto transposon carries a GAL4-regulated promoter expressing an exon encoding a FLAG-mCherry tag. Upon expression, the Hto exon can splice to a downstream genomic exon, generating a fusion transcript and tagged protein. RESULTS: Using rough-eye phenotypic screens,...
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