Article
A paper-based invasion assay: assessing chemotaxis of cancer cells in gradients of oxygen.
Biomaterials - 1 Jun 2015
Mosadegh Bobak, Lockett Matthew R, Minn Kyaw Thu, Simon Karen A, Gilbert Karl, Hillier Shawn, Newsome David, Li Howard, Hall Amy B, Boucher Diane M, Eustace Brenda K, Whitesides George M
Abstract excerpt
This work describes a 3D, paper-based assay that can isolate sub-populations of cells based on their invasiveness (i.e., distance migrated in a hydrogel) in a gradient of concentration of oxygen (O2). Layers of paper impregnated with a cell-compatible hydrogel are stacked and placed in a plastic holder to form the invasion assay. In most assays, the stack comprises a single layer of paper containing mammalian...
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