Article
Attenuated colicin-based screening to discover and create novel resistance genes.
Journal of microbiological methods - 1 May 2014
Futai Kazuki
Abstract excerpt
I formulated a systematic approach to screen for colicin resistance-associated genes by using an attenuated colicin mutant and demonstrated its utility in a screen of genes related to colicin E5 resistance. Screening of an Escherichia coli genome library revealed rstA as a partial resistance gene to colicin E5. Transcript expression of BtuB and OmpF, proteins responsible for translocation of nuclease E colicins,...
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