PCR Strategies for Complete Allele Calling in Multigene Families Using High-Throughput Sequencing Approaches.

Abstract excerpt

The characterization of multigene families with high copy number variation is often approached through PCR amplification with highly degenerate primers to account for all expected variants flanking the region of interest. Such an approach often introduces PCR biases that result in an unbalanced representation of targets in high-throughput sequencing libraries that eventually results in incomplete detection of the...

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• DNA
• DNA Copy Number Variations
• DNA Primers
• Exons
• Genes, MHC Class I