Article
Genotyping-by-sequencing (GBS): a novel, efficient and cost-effective genotyping method for cattle using next-generation sequencing.
PloS one - 1 Jan 2013
De Donato Marcos, Peters Sunday O, Mitchell Sharon E, Hussain Tanveer, Imumorin Ikhide G
Abstract excerpt
High-throughput genotyping methods have increased the analytical power to study complex traits but high cost has remained a barrier for large scale use in animal improvement. We have adapted genotyping-by-sequencing (GBS) used in plants for genotyping 47 animals representing 7 taurine and indicine breeds of cattle from the US and Africa. Genomic DNA was digested with different enzymes, ligated to adapters...
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