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Article

Use of a genetically encoded hydrogen peroxide sensor for whole cell screening of enzyme activity.

Protein engineering, design & selection : PEDS - 1 Mar 2015

Lim Joseph B, Sikes Hadley D

Abstract excerpt

We report the use of HyPer, a genetically encoded, fluorescent sensor that reacts with hydrogen peroxide (H2O2), in a novel screen to engineer enzymes for enhanced production of H2O2. We co-expressed HyPer with cytochrome P450 BM3 variants and, using HyPer's ratiometric signal, found variants that produce greater amounts of H2O2 than the wild-type enzyme through the leakage reaction. The screen avoids lysis...

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PMID: 25691762
DOI: 10.1093/protein/gzv003

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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