Article
A genetically encoded sensor for H2O2 with expanded dynamic range.
Bioorganic & medicinal chemistry - 1 Feb 2011
Markvicheva Kseniya N, Bilan Dmitry S, Mishina Natalia M, Gorokhovatsky Andrey Yu, Vinokurov Leonid M, Lukyanov Sergey, Belousov Vsevolod V
Abstract excerpt
Hydrogen peroxide is an important second messenger controlling intracellular signaling cascades by selective oxidation of redox active thiolates in proteins. Changes in intracellular [H(2)O(2)] can be tracked in real time using HyPer, a ratiometric genetically encoded fluorescent probe. Although...
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