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Article

Single genome amplification and standard bulk PCR yield HIV-1 envelope products with similar genotypic and phenotypic characteristics.

Journal of virological methods - 1 Mar 2015

Etemad Behzad, Ghulam-Smith Melissa, Gonzalez Oscar, White Laura F, Sagar Manish

Abstract excerpt

Recent studies suggest that single genome amplification (SGA) as compared to standard bulk PCR and virus stocks from 293T transfection versus short term passage in peripheral blood mononuclear cells (PBMC) yield a less biased representation of HIV-1 envelope characteristics. In 9 different subjects, genetic diversity, divergence, and population structure were not significantly different among SGA or bulk PCR...

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Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to env Gene Products, Human Immunodeficiency Virus

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Identifiers

PMID: 25681527
DOI: 10.1016/j.jviromet.2015.01.006
PMCID: PMC4394631

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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