Article
Catalytic stimulation by restrained active-site floppiness--the case of high density lipoprotein-bound serum paraoxonase-1.
Journal of molecular biology - 27 Mar 2015
Ben-David Moshe, Sussman Joel L, Maxwell Christopher I, Szeler Klaudia, Kamerlin Shina C L, Tawfik Dan S
Abstract excerpt
Despite the abundance of membrane-associated enzymes, the mechanism by which membrane binding stabilizes these enzymes and stimulates their catalysis remains largely unknown. Serum paraoxonase-1 (PON1) is a lipophilic lactonase whose stability and enzymatic activity are dramatically stimulated when associated with high-density lipoprotein (HDL) particles. Our mutational and structural analyses, combined with...
Topics
- Aryldialkylphosphatase
- Calcium
- Carboxylic Ester Hydrolases
- Catalysis
- Catalytic Domain
- Computer Simulation
- Crystallography, X-Ray
- Humans
- Hydrogen Bonding
- Kinetics
- Lipoproteins, HDL
- Models, Molecular
- Mutation