Article
In situ localization of N and C termini of subunits of the flagellar nexin-dynein regulatory complex (N-DRC) using SNAP tag and cryo-electron tomography.
The Journal of biological chemistry - 27 Feb 2015
Song Kangkang, Awata Junya, Tritschler Douglas, Bower Raqual, Witman George B, Porter Mary E, Nicastro Daniela
Abstract excerpt
Cryo-electron tomography (cryo-ET) has reached nanoscale resolution for in situ three-dimensional imaging of macromolecular complexes and organelles. Yet its current resolution is not sufficient to precisely localize or identify most proteins in situ; for example, the location and arrangement of components of the nexin-dynein regulatory complex (N-DRC), a key regulator of ciliary/flagellar motility that is...
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