Article
Genome-wide discovery of human splicing branchpoints.
Genome research - 1 Feb 2015
Mercer Tim R, Clark Michael B, Andersen Stacey B, Brunck Marion E, Haerty Wilfried, Crawford Joanna, Taft Ryan J, Nielsen Lars K, Dinger Marcel E, Mattick John S
Abstract excerpt
During the splicing reaction, the 5' intron end is joined to the branchpoint nucleotide, selecting the next exon to incorporate into the mature RNA and forming an intron lariat, which is excised. Despite a critical role in gene splicing, the locations and features of human splicing branchpoints are largely unknown. We use exoribonuclease digestion and targeted RNA-sequencing to enrich for sequences that traverse...
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