Article
Isolation and culture of primary osteocytes from the long bones of skeletally mature and aged mice.
BioTechniques - 1 Jun 2012
Stern Amber Rath, Stern Matthew M, Van Dyke Mark E, Jähn Katharina, Prideaux Matthew, Bonewald Lynda F
Abstract excerpt
The purpose of this work was to establish a methodology to enable the isolation and study of osteocytes from skeletally mature young (4-month-old) and old (22-month-old) mice. The location of osteocytes deep within bone is ideal for their function as mechanosensors. However, this location makes the observation and study of osteocytes in vivo technically difficult. Osteocytes were isolated from murine long bones...
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