Article
Variation in pre-PCR processing of FFPE samples leads to discrepancies in BRAF and EGFR mutation detection: a diagnostic RING trial.
Journal of clinical pathology - 1 Feb 2015
Kapp Joshua R, Diss Tim, Spicer James, Gandy Michael, Schrijver Iris, Jennings Lawrence J, Li Marilyn M, Tsongalis Gregory J, de Castro David Gonzalez, Bridge Julia A, Wallace Andrew, Deignan Joshua L, Hing Sandra, Butler Rachel, Verghese Eldo, Latham Gary J, Hamoudi Rifat A
Abstract excerpt
AIMS: Mutation detection accuracy has been described extensively; however, it is surprising that pre-PCR processing of formalin-fixed paraffin-embedded (FFPE) samples has not been systematically assessed in clinical context. We designed a RING trial to (i) investigate pre-PCR variability, (ii) correlate pre-PCR variation with EGFR/BRAF mutation testing accuracy and (iii) investigate causes for observed variation....
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