Article
[Construction of an activation tagging library of Arabidopsis and cloning for mutant genes].
Zhi wu sheng li yu fen zi sheng wu xue xue bao = Journal of plant physiology and molecular biology - 1 Oct 2005
Li Zhi-Miao, Zhang Hai-Kuo, Cao Jia-Shu, He Zu-Hua
Abstract excerpt
Activation tagging is an important strategy in plant genomics by generating gain-of-function mutants. In this work, a library of Arabidopsis mutants was constructed by in planta transformation mediated by Agrobacterium tumefaciens containing an activation tagging vector pSKI015 with herbicide Basta as a selection marker (Fig. 1). Among 20000 independent transformants, 38 lines, i.e. about 0.2% of T(1) progeny,...
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