Article
Single-nucleotide polymorphism discrimination using high-resolution melting analysis for the genotyping of Bacillus anthracis.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2015
Derzelle Sylviane
Abstract excerpt
High-resolution melting (HRM) is a post-PCR technique that determines with high precision the melt profile of PCR products using a new generation of double-stranded DNA-binding dyes and accurate fluorescence data acquisition over small temperature increments. The method can be used to interrogate small sets of single-nucleotide polymorphisms (SNPs). Here, we describe a simple and cost-effective HRM-based method...
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