Article
Cost-effective interrogation of single nucleotide polymorphisms using the mismatch amplification mutation assay and capillary electrophoresis.
Electrophoresis - 1 Dec 2010
Price Erin P, Matthews Molly A, Beaudry Jodi A, Allred Jonathan L, Schupp James M, Birdsell Dawn N, Pearson Talima, Keim Paul
Abstract excerpt
The ability to characterize SNPs is an important aspect of many clinical diagnostic, genetic and evolutionary studies. Here, we designed a multiplexed SNP genotyping method to survey a large number of phylogenetically informative SNPs within the genome of the bacterium Bacillus anthracis. This novel method, CE universal tail mismatch amplification mutation assay (CUMA), allows for PCR multiplexing and automatic...
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