Article
High-resolution melting PCR analysis for rapid genotyping of Burkholderia mallei.
Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases - 1 Sept 2018
Girault G, Wattiau P, Saqib M, Martin B, Vorimore F, Singha H, Engelsma M, Roest H J, Spicic S, Grunow R, Vicari N, De Keersmaecker S C J, Roosens N H C, Fabbi M, Tripathi B N, Zientara S, Madani N, Laroucau K
Abstract excerpt
Burkholderia (B.) mallei is the causative agent of glanders. A previous work conducted on single-nucleotide polymorphisms (SNP) extracted from the whole genome sequences of 45 B. mallei isolates identified 3 lineages for this species. In this study, we designed a high-resolution melting (HRM) method for the screening of 15 phylogenetically informative SNPs within the genome of B. mallei that subtype the species...
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