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Article

Characterization of genetic diversity of Bacillus anthracis in France by using high-resolution melting assays and multilocus variable-number tandem-repeat analysis.

Journal of clinical microbiology - 1 Dec 2011

Derzelle S, Laroche S, Le Flèche P, Hauck Y, Thierry S, Vergnaud G, Madani N

Abstract excerpt

Using high-resolution melting (HRM) analysis, we developed a cost-effective method to genotype a set of 13 phylogenetically informative single-nucleotide polymorphisms (SNPs) within the genome of Bacillus anthracis. SNP discrimination assays were performed in monoplex or duplex and applied to 100 B. anthracis isolates collected in France from 1953 to 2009 and a few reference strains. HRM provided a reliable and...

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Anthrax
Bacillus anthracis
Environmental Microbiology
France
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Molecular Typing

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PMID: 21998431
DOI: 10.1128/JCM.05439-11
PMCID: PMC3232934

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