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Article

A system for the continuous directed evolution of proteases rapidly reveals drug-resistance mutations.

Nature communications - 30 Oct 2014

Dickinson Bryan C, Packer Michael S, Badran Ahmed H, Liu David R

Abstract excerpt

The laboratory evolution of protease enzymes has the potential to generate proteases with therapeutically relevant specificities and to assess the vulnerability of protease inhibitor drug candidates to the evolution of drug resistance. Here we describe a system for the continuous directed evoluti...

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Identifiers

PMID: 25355134
DOI: 10.1038/ncomms6352
PMCID: PMC4215169

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 4 Sept 2026. Prepared record publication-25355134-2026-09-04.2. Manifest publication-graph-cohort-2026-09-04.2.

Source receipt: PubMed via Helena Literature Corpus, retrieved 4 Sept 2026.

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