Article
Characterization of the mutagenic spectrum of 4-nitroquinoline 1-oxide (4-NQO) in Aspergillus nidulans by whole genome sequencing.
G3 (Bethesda, Md.) - 27 Oct 2014
Downes Damien J, Chonofsky Mark, Tan Kaeling, Pfannenstiel Brandon T, Reck-Peterson Samara L, Todd Richard B
Abstract excerpt
4-Nitroquinoline 1-oxide (4-NQO) is a highly carcinogenic chemical that induces mutations in bacteria, fungi, and animals through the formation of bulky purine adducts. 4-NQO has been used as a mutagen for genetic screens and in both the study of DNA damage and DNA repair. In the model eukaryote Aspergillus nidulans, 4-NQO-based genetic screens have been used to study diverse processes, including gene regulation,...
Topics
- 4-Nitroquinoline-1-oxide
- Adenine
- Aspergillus nidulans
- DNA Damage
- Genome, Fungal
- Guanine
- High-Throughput Nucleotide Sequencing
- Mutagenesis
- Phenotype
- Point Mutation
- Sequence Analysis, DNA