Enhanced production of L-sorbose from D-sorbitol by improving the mRNA abundance of sorbitol dehydrogenase in Gluconobacter oxydansWSH-003

Abstract excerpt

BACKGROUND: Production of L-sorbose from D-sorbitol by Gluconobacter oxydans is the first step to produce L-ascorbic acid on industrial scale. The sldhAB gene, which encodes the sorbitol dehydrogenase (SLDH), was overexpressed in an industrial strain G. oxydans WSH-003 with a strong promoter, P tufB . To enhance the mRNA abundance, a series of artificial poly(A/T) tails were added to the 3'-terminal of sldhAB...

Topics

Share this publication in a Topic to start or enrich a Post.

• Share to Biochemical Acid Research Studies
• Share to Diet, Metabolism, and Disease
• Share to Microbial metabolism and enzyme function