Article
Production of copolyesters of 3-hydroxybutyrate and medium-chain-length 3-hydroxyalkanoates by E. coli containing an optimized PHA synthase gene.
Microbial cell factories - 14 Sept 2012
Gao Xue, Yuan Xiao-Xi, Shi Zhen-Yu, Guo Ying-Ying, Shen Xiao-Wen, Chen Jin-Chun, Wu Qiong, Chen Guo-Qiang
Abstract excerpt
BACKGROUND: Microbial polyhydroxyalkanoates (PHA) are biopolyesters consisting of diverse monomers. PHA synthase PhaC2Ps cloned from Pseudomonas stutzeri 1317 is able to polymerize short-chain-length (scl) 3-hydroxybutyrate (3HB) monomers and medium-chain-length (mcl) 3-hydroxyalkanoates (3HA) with carbon chain lengths ranging from C6 to C12. However, the scl and mcl PHA production in Escherichia coli expressing...
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