Article
Two-stage fermentation process for alginate production by Azotobacter vinelandii mutant altered in poly-beta-hydroxybutyrate (PHB) synthesis.
Journal of applied microbiology - 1 Jan 2010
Mejía M A, Segura D, Espín G, Galindo E, Peña C
Abstract excerpt
AIMS: A two-stage fermentation strategy, based on batch cultures conducted first under non-oxygen-limited conditions, and later under oxygen-limited conditions, was used to improve alginate production by Azotobacter vinelandii (AT6), a strain impaired in poly-beta-hydroxybutyrate (PHB) production. METHODS AND RESULTS: The use of sucrose as carbon source, as well as a high oxygen concentration (10%), allowed to...
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