Article
Increasing the stability of sacB transcript improves levansucrase production in Bacillus subtilis.
Letters in applied microbiology - 1 Jan 2005
Daguer J P, Chambert R, Petit-Glatron M F
Abstract excerpt
AIMS: To develop a strategy to increase the stability of transcripts of structural genes expressed under the control of sacR, the leader region of Bacillus subtilis levansucrase gene. METHODS AND RESULTS: Insertion of Shine Dalgarno like sequences in the 5'-untranslated sacR region controlling the expression of sacB. Depending on the number of stabilizing sequences inserted and the position of these sequences...
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