Article
The lack of trade-off between conformational stability and binding affinity in a nanobody with therapeutic potential for a misfolding disease.
International journal of biological macromolecules - 1 Jan 2025
Gómez-Mulas Atanasio, Naganathan Athi N, Pey Angel L
Abstract excerpt
To improve protein pharmaceuticals, we need to balance protein stability and binding affinity with in vivo efficiency. We have recently developed a nanobody (NB-AGT-2) against the alanine:glyoxylate aminotransferase with high stability (Tm ∼ 86 °C) that may be useful to treat a misfolding disease called primary hyperoxaluria type 1. In this work, we characterize the relationships between protein stability and...
Topics
- Single-Domain Antibodies
- Protein Binding
- Protein Stability
- Thermodynamics
- Humans
- Mutation
- Protein Conformation
- Proteostasis Deficiencies
- Models, Molecular