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Article

Long-range distance measurements in proteins at physiological temperatures using saturation recovery EPR spectroscopy.

Journal of the American Chemical Society - 29 Oct 2014

Yang Zhongyu, Jiménez-Osés Gonzalo, López Carlos J, Bridges Michael D, Houk K N, Hubbell Wayne L

Abstract excerpt

Site-directed spin labeling in combination with EPR is a powerful method for providing distances on the nm scale in biological systems. The most popular strategy, double electron-electron resonance (DEER), is carried out at cryogenic temperatures (50-80 K) to increase the short spin-spin relaxation time (T2) upon which the technique relies. A challenge is to measure long-range distances (20-60 Å) in proteins near...

Topics

Amino Acid Sequence
Bacteriophage T4
Binding Sites
Copper
Electron Spin Resonance Spectroscopy
Models, Molecular
Muramidase
Mutation
Nitrogen Oxides
Peptides
Protein Structure, Secondary

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Proteins

Quantum Theory

Rotation

Spin Labels

Temperature

Source

Open in PubMed Open full text

Identifiers

PMID: 25290172
DOI: 10.1021/ja5083206
PMCID: PMC4227719

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