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Article

Long-range distance measurements in proteins at physiological temperatures using saturation recovery EPR spectroscopy.

Journal of the American Chemical Society - 29 Oct 2014

Yang Zhongyu, Jiménez-Osés Gonzalo, López Carlos J, Bridges Michael D, Houk K N, Hubbell Wayne L

Abstract excerpt

Site-directed spin labeling in combination with EPR is a powerful method for providing distances on the nm scale in biological systems. The most popular strategy, double electron-electron resonance (DEER), is carried out at cryogenic temperatures (50-80 K) to increase the short spin-spin relaxation time (T2) upon which the technique relies. A challenge is to measure long-range distances (20-60 Å) in proteins near...

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Identifiers

PMID: 25290172
DOI: 10.1021/ja5083206
PMCID: PMC4227719

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