Article
Gadolinium tagging for high-precision measurements of 6 nm distances in protein assemblies by EPR.
Journal of the American Chemical Society - 13 Jul 2011
Yagi Hiromasa, Banerjee Debamalya, Graham Bim, Huber Thomas, Goldfarb Daniella, Otting Gottfried
Abstract excerpt
Double electron-electron resonance (DEER) distance measurements of a protein complex tagged with two Gd(3+) chelates developed for rigid positioning of the metal ion are shown to deliver outstandingly accurate distance measurements in the 6 nm range. The accuracy was assessed by comparison with modeled distance distributions based on the three-dimensional molecular structures of the protein and the tag and...
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