Gadolinium tagging for high-precision measurements of 6 nm distances in protein assemblies by EPR.

doi:10.1021/ja204415w

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Article

Gadolinium tagging for high-precision measurements of 6 nm distances in protein assemblies by EPR.

Journal of the American Chemical Society - 13 Jul 2011

Yagi Hiromasa, Banerjee Debamalya, Graham Bim, Huber Thomas, Goldfarb Daniella, Otting Gottfried

Abstract excerpt

Double electron-electron resonance (DEER) distance measurements of a protein complex tagged with two Gd(3+) chelates developed for rigid positioning of the metal ion are shown to deliver outstandingly accurate distance measurements in the 6 nm range. The accuracy was assessed by comparison with modeled distance distributions based on the three-dimensional molecular structures of the protein and the tag and...

Topics

Animals
Crystallography, X-Ray
Electron Spin Resonance Spectroscopy
Gadolinium
Heat-Shock Proteins
Humans
Mutation
Nuclear Magnetic Resonance, Biomolecular
Protein Structure, Secondary
Rats

Source

Open in PubMed

Identifiers

PMID: 21661728
DOI: 10.1021/ja204415w

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 31 Aug 2026.

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