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Article

Tethered spectroscopic probes estimate dynamic distances with subnanometer resolution in voltage-dependent potassium channels.

Biophysical journal - 17 Dec 2013

Jarecki Brian W, Zheng Suqing, Zhang Leili, Li Xiaoxun, Zhou Xin, Cui Qiang, Tang Weiping, Chanda Baron

Abstract excerpt

Measurements of inter- and intramolecular distances are important for monitoring structural changes and understanding protein interaction networks. Fluorescence resonance energy transfer and functionalized chemical spacers are the two predominantly used strategies to map short-range distances in living cells. Here, we describe the development of a hybrid approach that combines the key advantages of spectroscopic...

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Identifiers

PMID: 24359744
DOI: 10.1016/j.bpj.2013.11.010
PMCID: PMC3882458

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