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Article

F plasmid TraF and TraH are components of an outer membrane complex involved in conjugation.

Journal of bacteriology - 1 Mar 2010

Arutyunov Denis, Arenson Barbara, Manchak Jan, Frost Laura S

Abstract excerpt

F plasmid TraF and TraH are required for F pilus assembly and F plasmid transfer. Using flotation sucrose density gradients, we found that TraF and TraH (as well as TraU and TraW) localized to the outer membrane in the presence of the complete F transfer region, especially TraV, the putative anchor. Mutational analysis of TraH revealed two domains that are important for its function and possible interaction with...

Topics

Amino Acid Sequence
Bacterial Proteins
Cell Membrane
Conjugation, Genetic
Conserved Sequence
Escherichia coli
Escherichia coli Proteins
F Factor
Molecular Sequence Data
Mutation
Nuclear Proteins

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Protein Structure, Tertiary

Protein Transport

Source

Open in PubMed Open full text

Identifiers

PMID: 20081027
DOI: 10.1128/JB.00726-09
PMCID: PMC2832511

Provenance

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F plasmid TraF and TraH are components of an outer membrane complex involved in conjugation.PMID 20081027Read ↗

Characterization of the F-plasmid conjugative transfer gene traU.Semantic proximity Read ↗

Characterization of the F plasmid mating aggregation gene traN and of a new F transfer region locus trbE.Semantic proximity Read ↗

Mutational analysis of TraM correlates oligomerization and DNA binding with autoregulation and conjugative DNA transfer.Semantic proximity Read ↗

A rapid screen for functional mutants of TraM, an autoregulatory protein required for F conjugation.Semantic proximity Read ↗

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F-like type IV secretion systems encode proteins with thioredoxin folds that are putative DsbC homologues.Semantic proximity Read ↗

Structural basis of cooperative DNA recognition by the plasmid conjugation factor, TraM.Semantic proximity Read ↗

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